AHCSDT403A
Perform an Anguina test on annual ryegrass seed

This Unit covers testing seed of annual ryegrass for the presence of Nematode Gall (Anguina tenuis).

Application

This Unit applies to senior seed analysts working within enterprise guidelines. In general, they oversee the preparation of samples for testing; conduct and supervise specified tests in accordance with enterprise requirements; interpret and report results; maintain record-keeping systems; and have responsibility for overall laboratory operations, the supervision of staff, and implementation of quality assurance procedures.


Prerequisites

Nil.


Elements and Performance Criteria

1. Prepare for Anguina testing

1.1 Test request is reviewed to identify samples to be tested, required test methods, equipment, and staff to be involved in the test.

1.2 Equipment and working space are cleaned and calibrated.

1.3 The working sample or sub-samples are prepared using mixing and dividing processes.

1.4 Sample details are correctly recorded on worksheets and sample packets.

2. Inspect seed samples

2.1 A prescribed sample of ryegrass seed is prepared and inspected on a diaphanoscope for the presence of galls.

2.2 Any galls present, are separated into yellow and black galls, counted and reported separately.

2.3 If galls are not present, then further samples of six grams (6gms) are inspected to a total of sixty grams (60gms) of seed.

3. Examine galls

3.1 Galls are soaked in water for prescribed period.

3.2 Galls are dissected and examined under a microscope for the presence of nematodes.

3.3 The presence of nematodes is recorded.

4. Report results

4.1 Testing procedures carried out by analysts are reviewed.

4.2 Where no galls are found on the sixty grams (60gms) of seed, a report of no galls found is issued.

4.3 Where galls have been detected containing nematodes, these are reported as number of galls per sample weight.

Required Skills

Required skills include:

Ability to:

• plan work sequences

• inspect seed using a diaphanoscope

• dissect galls and using a microscope

• identify nematodes

• record and reporting of results of tests

• follow enterprise guidelines and standard operating procedures.

Required knowledge includes:

Knowledge of:

• seed biology

• basic identification of nematodes

• recording and reporting protocols

• enterprise standard operating procedures (SOPs)

• relevant health, safety and environment requirements.

Evidence Required

The evidence guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package.

Overview of assessment

Critical aspects for assessment and evidence required to demonstrate competency in this Unit

Evidence of the ability to:

plan and delegate testing tasks

inspect seed samples using a diaphanoscope

identify galls on ryegrass seed

use a microscope to detect nematodes.

Context of and specific resources for assessment

Competency requires the application of work practices under work conditions. Selection and use of resources for some worksites may differ due to the regional or enterprise circumstances.

Method of assessment

This Unit should be assessed together with core Units and other Units of Competency relevant to the function or work role.

Guidance information for assessment

To ensure consistency of performance, competency should be demonstrated on more than one occasion over a period of time in order to cover a variety of circumstances, cases and responsibilities, and where possible, over a number of assessment activities.


Range Statement

The range statement relates to the Unit of Competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included.

Galls may include:

black and yellow galls.


Sectors

Seed Testing


Employability Skills

This Unit contains employability skills.


Licensing Information

Not applicable.